Apoptosis inhibitor 3 (API3) is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2.
Similar to API1, API3 inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE.
API3 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7.
Applications:Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlImmunofluorescence: 1:100-1:200Immunocytochemistry: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.
Specificity：Recognizes human BIRC4. Species sequence homology: mouse (88%), rat (90%), rabbit (94%) and porcine (92%).
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa75-226 of human BIRC4.
