VPS35, otherwise known as MEM3, is a ubiquitously expressed member of the vacuolar protein sorting (VPS) family, and vital component of the multimeric retromer complex, involved in the retrieval of lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans Golgi network.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.01-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human VPS35. Species sequence homology: bovine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide C-SPESEGPIYEGLIL from the C-terminus of human VPS35.
