84-1218-66　［取扱停止］TLR9 (Toll-like Receptor 9, CD289, UNQ5798/PRO19605) 50ul　T8050-95K

特徴

• TLR9 is a 145-150kD member of the Toll-like receptor family of molecules.
• It is expressed by colonic epithelium, CD123+ plasmacytoid dendritic cells, and transitional B cells, and responds to unmethylated DNA CpG motifs that exhibit either a GTCGTT sequence (in human), or a GACGTT sequence (in mouse).
• TLR9 is found in the ER, and translocates to either the cell membrane, or to lysosomes where it binds bacterial DNA.
• Precursor human TLR9 is a type I transmembrane protein 1032aa in length.
• It possesses a 793aa extracellular region that contains 26 LRRs (aa26-818), plus a 193aa cytoplasmic domain.
• The full-length 150kD form is suggested to be ligand-binding but nonsignaling.
• The active form is believed to be an 80kD cleavage product found in the endosome compartment.
• There are multiple splice forms.
• One contains a deletion of aa2-16, a second possesses an alternate start site at aa58, while a third and fourth show alternative start sites aa23 and 24 upstream of the standard site.
• Over aa64-189, human TLR9 shares 76aa identity with mouse TLR9.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.