SHP2, a NT2 non-receptor protein tyrosine phosphatases, contains two tandem SRC homology (SH2) domains at the N-terminus of the catalytic domain.
Based on crystallographic studies, the SH2 domain is believed to function as a conformational switch which can bind and inhibit the phosphatase or bind phosphoprotein and activate the enzyme.
SHP2 is thought to link growth factor receptors and other signalling proteins via protein-tyrosine phosphorylation.
It may play a role in mammalian budding morphogenesis and immune response.
Missense mutations in the SHP2 gene have been linked to Noonan Syndrome.
Additionally, SHP2 is present in diverse neurons and is thought to play a role in abroad spectrum of neuronal responses, including neuronal survival.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 1-3ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human PTPN11. Species sequence homology: bovine, mouse, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide C-YENVGLMQQQKSFR from the C-terminus of human PTPN11.
