UCHL1 is a member of family of proteins that hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer.
The expression of UCHL1 is highly specific to neurons and to cell of the diffuse neuroendocrine system and their tumors.
UCHL1 exhibits both and hydrolase and a ligase activity.
The S18Y variant of UCHL1, that has reduced ligase activity, has been linked to susceptibility for Parkinson's disease.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:5000Western Blot: 1:500-1:1000Immunohistochemistry (FFPE): 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of total human PGP9.5. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa171-220 from human PGP9.5
