PRDM16, also known as Mel1, is a zinc finger protein that plays a role in the differentiation of brown adipose tissue, which plays a role in dissipating heat in response to cold or excess feeding.
PRDM16 works together with CEBPB to regulate the differentiation of myoblastic precursors into brown adipose cells.
PRDM16 is also a repressor of TGF beta signalling, interacting with SKI to promote the recruitment of the SMAD3-HDAC1 complex to the promoter of TGF beta target genes.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PRDM16. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 17aa from human PRDM16 at C-terminus.
