NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline.
Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities.
Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:250ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.
Specificity:Recognizes mouse iNOS. Species sequence homology: human and rat.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Mo
Immunogen:Synthetic peptide corresponding to the C-terminal of mouse iNOS.