MEKK7, known as TAK1 for transforming growth factor (TGF) beta-activated kinase-1, is a MLK (MAP3K) type protein kinase which is associated with several signaling pathways.
The protein contains a N-terminal protein kinase domain preceeded by a short regulatory motif and followed by a C-terminal extension of unknown function.
TAK1 is activated by TGF beta and tumor necrosis factor (TNF).
Further, TAK1 binding proteins, TAB-1 and -2, bind to the N-terminal regulatory motif and couple TAK1 to additional upstream signals, including the human X chromosome-linked inhibitor of apoptosis.
TAK1 regulates the JNK cascade by phosphorylating MKK4/MAP2K4 and p38 cascades by phosphorylating MEK3/MAP2K3 and MKK6/MAP2K6.
TAK1 also has been implicated in interleukin-1 (IL-1) signaling to JNKs via direct activation by IL-1 or by forming a complex with TRAF6, TAB1 and TAB2.
At least four protein isoforms, resulting from alternatively spliced transcript variants, have been identified - 491, 518, 579 and 606aa.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:40,000Western Blot: 1:500-1:1000Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MAP3K7. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human MAP3K7.
