Matriptase, also called MT-SP1, TADG-15 and ST14, is a member of the type II transmembrane serine protease family and is expressed on most human epithelia as an 80-90kD acell surface glycoprotein.
Processing, activation and shedding of the C-terminal 26kD peptidase S1 domain is complex and requires coexpression with its inhibitor, HAI-1.
Matriptase activity is critical to epidermal barrier formation.
It is overexpressed in a variety carcinomas.
Human and mouse matriptase share 86% aa identity within the peptidase S1 domain.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000Western Blot: 1:500-1:1000Immunofluorescence: 1:100-1:500Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ST14.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human ST14.
