Applications:Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:300Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlImmunocytochemistry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：125ug
Host：sheep
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4.
Specificity：Recognizes bovine Histone H3. Minimal cross-reactivity with linker histone H1 and no cross-reactivity with histone core proteins H2A, H2B, or H4. Species sequence homology: human.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Bo
Immunogen：Intact calf histone H3 at 15.4kD, 136aa, complexed with RNA.
