Grb2 is an adaptor protein which has no enzymatic activity; however, Grb2 plays an important role in signaling cascades by physically linking other proteins through its phosphotyrosine-binding SH2 domain and its proline-binding SH3 domain.
Grb2 associates with the guanine nucleotide exchange factor Sos via its SH3 domain.
In response to cell stimulation with growth factors, growth factor receptors are autophosphorylated on cytoplasmic tyrosine residues.
Grb2's association with these tyrosine residues via its SH2 domain permits the localization of Sos with the growth factor receptor near the cell membrane.
Sos is then able to activate the membrane associated protein Ras which in turn activates the MAPK cascade and influences gene expression.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 15mM sodium azide, 0.2% BSA.
Specificity：Recognizes human GRB2.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa198-217 from C-terminus of human Grb2.
