Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively.
GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles.
The two enzymes are homodimeric and show close homology.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:sheep
Source Antibody:porcine
Grade:Purified
Purity:Purified
Form:Supplied as a liquid in 0.015M PBS, pH 7.2, 0.09% sodium azide.
Specificity:Recognizes porcine GOT2.
Isotype:IgG
Calc Applications Abbrev:IF IHC IP WB
Calc Crossreactivity:Po
Immunogen:Porcine GOT2 Species sequence homology: human.