Fibrinogen is the main protein of blood coagulation system.
It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma.
All chains are connected with each other by a number of disulfide bonds.
Fibrinopeptides A (1-16aa) and B (1-17aa) are released by thrombin from the N terminal parts of alpha and beta chains, respectively.
In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot.
Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation.
Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases.
The normal fibrinogen concentration in plasma is about 3mg/ml.
The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases.
An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease.
All these facts make fibrinogen an important parameter in the diagnosis of cardiovascular diseases.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.05M sodium phosphate, 0.1M sodium chloride, 1mM EDTA, pH 6.6.
