Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions.
Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell.
Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development.
Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling.
A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.
Specificity：Recognizes rat GJA1. Species sequence homology: human and mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to a segment of the 3rd cytoplasmic domain of rat connexin 43.
