BTK, a BTK/TEC type kinase, is a key regulator of B cell development.
BTK interacts with the SRC homolog 3 domains of FYN, LYN, and HCK.
All of these protein tyrosine kinases are activated upon stimulation of B and T cell receptors.
Defects in the Bruton tyrosine kinase (BTK) gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement.
A mutation registry for X-linked agammaglobulinemia, called BTKbase, can be found at http://www.
uta.
fi/imt/bioinfo/BTKbase/.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:10,000Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.
Specificity：Recognizes human Btk. Species sequence homology: mouse (97%), rat (97%) and bovine (98%).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa91-387 from human Btk.
