ApoE is a 35-40kD protein component of serum chylomicrons, VLDL, and HDL particles.
It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain.
ApoE is important in fatty acid homeostasis and memory formation.
Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders.
Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:40,000Western Blot: 1:5000-1:40,000Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 75mM PBS, 75mM sodium chloride, 0.02% sodium azide, pH 7.2.
Specificity：Recognizes human APOE.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human APOE.
