Type 1 Angiotensin Receptors (AT1A and AT1B) mediate the major cardiovascular effects of angiotensin II, controlling blood pressure and volume by regulating cell growth, vascular contraction, inflammatory responses, and salt and water retention.
Stimulation of AT1 receptors by Ang II activates phospholipase C beta, resulting in increased intracellular calcium and inositol 1, 4, 5-triphosphate concentrations, and activatesthe mitogen-activated protein kinases, such as extracellular regulated kinases, by way of Src and Ras as well as the JAK/STAT pathways.
AT1 knock-out mice have lower blood pressure than wild type mice and lose normal pressor response to exogenous Ang II.
AT1B differs from AT1A in its mRNA tissue distribution and in the 5' untranslated region.
AT1 blockers have become widely used in the treatment of hypertension and may offer benefits other than blood pressure reduction.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (FFPE): 20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human AGTR1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 16aa, aa210-260, from near the center of human AGTR1.
