Thymosin beta 4 is a 5kD member of the B-thymosin family of molecules.
Members of this family range from 41-44aa in length, and possess an isoelectric point that lies between pH 4.0-7.0 a-thymosins have values less than 4.0.
Multiple cell types produce TB4, either constitutively, or after stimulation.
They include platelets, endothelial cells, neutrophils, astrocytes and macrophages.
TB4 is both a secreted and intracellular molecule.
The secreted form contributes to wound healing and angiogenesis, and may act on ATPase.
Intracellularly, it forms a 1:1 complex with G-actin and blocks F-actin polymerization.
This regulates the availablility of actin monomers for filament formation and subsequent cell migration.
Mature human TB4 is 43aa in length aa2-44.
It contains an actin-binding site aa17-23, one acetylated Ser and five acetylated lysines (4; 12; 26; 32; 39) and one phosphorylation site at Thr23.
TB4 undergoes proteolytic processing to generate an N-terminal acetylated peptide aa2-5: SerAspLysPro.
Mature human TB4 is identical to mouse TB14 in aa sequence, and it shares 74% aa identity with its human family member TB10.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human TB4. Species sequence homology: Recombinant human Thymosin b10 and rhThymosin b16 (5%).
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa2-44 from human TB4 expressed in E.coli.
