84-1216-43　TDRD1 (Tudor Domain-containing Protein 1, Cancer/Testis Antigen 41.1, CT41.1) 100ug　T5152-12

特徴

• DRD is a 120-140kD member of the Tudor family of proteins.
• It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression.
• TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element- induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively.
• This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex.
• Human TDRD1 is 1180aa in length.
• It contains one MYND-type Zn finger region aa170-206, and four methyl-binding Tudor domains aa312-1048.
• There are four potential splice variants with predicted molecular weights ranging between 79kD and 133kD.
• One contains an alternate start site at Met404, another contains an alternative start site at Met22, accompanied by deletions of aa554-610 and 775-831, a third shows a 10aa substitution for 1180aa, while a fourth contains deletions of aa328-375, 554-610 and 1059-1134.
• Over aa837-968, human TDRD1 shares 66% aa identity with mouse TDRD1.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.