SCD1 (Stearoyl-CoAdesaturase 1; also AcylCoA desaturase, fatty acid desaturase, and Delta9 desaturase) is a 37-40kD member of the fatty acid desaturase family of enzymes.
It is an ER-embedded protein that is expressed by multiple cell types, including adipocytes, hepatocytes, macrophages, endothelial and sebaceous gland cells.
SCD1 catalyzes the formation of mono-unsaturated fatty acids from saturated fatty acids.
It does so by generating a double bond between the C9 and C10 carbons of dietary and/or endogenously synthesized fatty acids.
This creates either palmitoleic or oleic acid, 2 fatty acids that are optimally suited for either storage or inclusion into phospholipids.
It also removes a potential source of inflammation, as saturated fatty acids are known to activate TLRs with the subsequent onset of inflammation.
Human SCD1 is a 4-transmembrane (TM), 359aa protein.
It contains a 71aa cytoplasmic N-terminus, followed by two TM segments (aa72-119) and an extended cytoplasmic region (aa120-216) that possesses three utilized Ser/Thr phosphorylation sites, two additional TM segments (aa217-273), and a C-terminal cytoplasmic tail (aa274-359) that contains most of the catalytic region.
There is one potential isoform variant that shows 13aa substitution for aa295-359.
Over aa141-221, human SCD1 shares 95% aa sequence identity with mouse SCD1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human SCD-1. Species Crossreactivity: Recombinant human SCD5, recombinant mouse SCD1 (<5%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant corresponding to aa141-221 from human SCD1 expressed in E.coli.
