SMAD9 is a 55-60kD R-Group member of the dwarfin/SMAD family of proteins.
It is expressed by BMP-responsive cells, and serves to transmit information from the BMP receptor (BMPR) to the nucleus.
In particular, following BMPR activation, SMAD9 is phosphorylated by the BMPR on its C-terminus, promoting heterodimerization with SMAD4 and its translocation into the nucleus.
Here, SMAD9 binds to DNA and participates in gene activation.
Human SMAD9 is 467aa in length.
It contains a DNA-binding domain termed MH1 aa16-140 plus a receptor/transcription factor binding domain termed MH2 aa273-467.
The C-terminus contains two phosphorylation sites at aa465 and aa467.
There is one splice form that shows a deletion of aa224-260.
Over aa158-223, human and mouse SMAD9 share 82% identy in aa sequence.
Applications:Suitable for use in Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 5-15ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human SMAD9.
Isotype：IgG
Calc Applications Abbrev：IC IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa158-223 from human SMAD9 expressed in E.coli.
