Siglecs are I-type lectins that belong to the immunoglobulin superfamily.
They are characterized by an N-terminal Ig-like V-set domain which mediates sialic acid binding, followed by a varying numbers of Ig-like C2-set domains.
Siglec-3 and Siglecs-5-13 constitute the CD33/Siglec-3 related group, which are defined by their sequence homology and differential expression in the hematopoietic system.
In humans, Siglec-11 is expressed in tissue macrophages, brain microglia, and inflammatory site monocytes.
Strong microglial expression is specific to humans, as it is less prominent or absent in chimpanzees and orangutans.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: Neat.
10ul labels up to 1x10e6 cells
Optimal dilutions to be determined by the researcher.
Excitation:488nm
Emission:565-605nm
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Siglec-11.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asn17-His543 (Glu84Ala, Lys145Gln) from human Siglec-11 expressed in CHO cell line.
