84-1216-34　RGS8 (Regulator of G-protein Signaling 8) 100ug　R1996-19G

特徴

• RGS8 is a 22-23kD (predicted) member of the B/R4 subfamily, RGS family of proteins.
• It is expressed in NK cells and neurons, particularly cerebellar Purkinje cells, and is known to regulate GPCR signaling.
• When GPCRs are activated, a GPCR cytoplasmic domain associated GABG heterotrimer dissociates in response to GTP binding.
• This generates GA :GTP and GBG dimers that initiate downstream signaling.
• GA has GTPase activity, and when GTP is hydrolyzed, the trimer reforms and stops signaling.
• RGS8 potentiates GA GTP hydrolase activity, particularly when associated with M1 muscarinic acetylcholine receptors.
• Human RGS8 is 180aa in length, and is characterized by the presence of one RGS domain aa56-171, and an N-terminal GPCR binding motif aa6-9.
• There is one splice variant that shows a 27aa substitution for aa1-9.
• Expression of this variant may negatively impact its regulation of GA-coupled responses.
• Over aa10-76, human RGS8 shares 98% aa identity with mouse RGS8.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.