PSMA1 (Proteosome subunit alpha type1; also 30kD prosomal protein/PROS30, HC2, proteasome component C2/PSC2, PSMA1/a6, and NU) is a 30kD member of the peptidase T1A family of enzymes.
It is widely expressed, and found in both cytoplasm and nucleus.
Short-lived intracellular molecules (typically proteins) are enzymatically degraded by the 26S proteosome.
This is a multisubunit 3D complex that is over 2000kD in size, and recognizes previously ubiquitinated proteins.
The middle of this 26S complex is shaped like a barrel with four staves that run circumferentially rather than longitudinally.
Each stave contains seven subunits, with B type subunits generating the two center staves, and a-type subunits comprising the outer, or flanking staves.
The function of the barrel, also known as the 20S protease core "particle", is to enzymatically cleave substrates that enter its chamber.
For proteins, this is done by b-type subunits.
The 26S complex also cleaves mRNA, and this is mediated by a-type subunits.
PSMA 1/a6 does not cleave mRNA, but it does positively regulate PSMA5/a5 catalytic activity.
Notably, PSMA1 has also been reported to bind to LPS.
Human PSMA1 is 263aa in length.
It contains an acetylated Met at position #1, plus three utilized phosphorylation sites at 6aa, 11aa, and 16aa.
There are at least four potential isoform variants.
Three utilize alternative start sites.
One shows a start site at aa140, while a second and third initiates translation at sites 6 and 43aa upstream of the standard site, respectively.
A fourth isoform possess a 1aa substitution for aa115-263.
Over aa16-263, human PSMA1 shares 98% aa sequence identity with mouse PSMA1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human PSMA1. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant corresponding to aa16-263 from human PSMA1, expressed in E.coli.
