84-1216-27　Phox2b (Paired Mesoderm Homeobox Protein 2B, Neuroblastoma Phox, NBPhox, PHOX2B Homeodomain Protein, Paired-like Homeobox 2B, PMX2B) 100ug　P4079-03D

特徴

• PHOX2B is a 33-35kD member of the paired homeobox family of transcription factors.
• It is not actually a mesoderm-associated protein, but is instead apparently restricted to neuronal precursors and mature neurons.
• It is found in glutamatergic neurons of the NTS and in neurons that demarcate the respiratory chemoreception pathway.
• In addition, PHOX2B is essential to the development of noradrenergic adrenal chromaffin and sympathetic motor neurons.
• Human PHOX2B is 314aa in length.
• It contains one DNA binding homeobox domain aa98-157 and two poly-Ala sequences, one between aa159-167 and another between aa241-260.
• Alanine extensions involving anywhere from 5 to 11 Ala residues may exist.
• These change the nature of the PHOX2B molecule.
• While wild-type PHOX2B is nuclear, Ala extensions promote its retention in the cytoplasm with loss of activity.
• PHOX2B may potentially form oligomers with itself, or it paralogue, PHOX2A.
• Human and mouse PHOX2B are identical in aa sequence
• Applications:Suitable for use in Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.