84-1216-24　NFkB, p65 (Transcription Factor p65, Nuclear Factor NF-kappa-B p65 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B Cells 3, NFKB3, RELA) [KO Validated] 100ug　N2303-10L

特徴

• RelA belongs to a family of transcription factors (NFKB) that play a fundamental role in inflammatory and immune responses.
• The NFKB complex is composed of a heterodimer of a Rel family member (RelA, c-Rel, RelB) and either NFKB1 or NFKB2 subunits.
• RelA and NFKB1 are the most common heterodimeric pair.
• The NFKB complex is sequestered in the cytoplasm by inhibitory IKB proteins.
• Upon cellular activation, the ubiquitin-proteosome pathway degrades the IKB proteins allowing the NFKB complex to translocate to the nucleus and activate gene transcription.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunoprecipitation: 5ug labels 5 x10e6 cellsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.