Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins.
The rat NF155 cDNA encodes a 1240aa precursor that contains a 24aa signal sequence, a 1086aa extracellular domain (ECD), a 21aa transmembrane segment, and a 109aa cytoplasmic domain.
The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif.
A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain.
Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155.
NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier.
Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin.
NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction.
The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction.
In addition to distinct expression patterns, Neurofascin isoforms have different functional properties.
Applications:Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml using rat and mouse brain tissue and human cerebellum tissueImmunohistochemistry: 5-15ug/ml using perfusion fixed frozen rat brain (dorsal root ganglion)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：chicken
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes rat Neurofascin. Species Crossreactivity: human and mouse
Isotype：IgY
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to aa25-1031 from rat Neurofascin, expressed in NSO cells (NP_446361).
