84-1216-22　MEF2C (Myocyte-specific Enhancer Factor 2C) 100ug　M9769-97L

特徴

• MEF2C is a transcriptional activator that is a member of the MEF2 subfamily, MADS (MCM1, Agamous, Deficiens, Serum response factor) gene family of proteins.
• Although its predicted MW is 51kD, it runs anomalously at 57kD in SDS-PAGE.
• It is expressed in B cells, plus postmitotic neurons and skeletal muscle cells that are undergoing differentiation.
• Human MEF2C is 473aa in length.
• It contains a MADS box for dimerization aa3-57, a DNA binding domain aa58-86, a beta-domain that enhances transcription aa271-278, a gamma-domain that, when phosphorylated, promotes transcriptional repression aa390-399, and seven acetylation sites plus multiple Ser/Thr phosphorylation sites.
• Sumoylation occurs on Lys391, and proteolytic cleavage of MEF2C occurs in neurons between aa432-433.
• Multiple splice forms exist.
• Either individually or in combination, there may be a deletion of aa271-278, 368-399 or 87-134, a 46aa substitution for aa107-134 and 87-134, and an alternative start site 35aa upstream of the standard site.
• Over aa135-239, human MEF2C shares 96% aa identity with mouse MEF2C.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.