84-1216-21　［取扱停止］MYF5 (Myogenic Factor 5, Myf-5, Myogenic Regulatory Factor 5, Class C Basic Helix-loop-helix Protein 2, bHLHc2) 100ug　M9761-01B

特徴

• MYF-5 is a 37-39kD transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors.
• It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1.
• Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype.
• Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype.
• MYF-5 forms heterodimers with multiple molecules.
• When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active.
• Phosphorylation regulates MYF-5 stability.
• Human MYF-5 is 255aa in length.
• It contains one myogenic basic region aa1-83 followed by an HLH motif aa96-135.
• Full-length human MYF-5 aa1-255 shares 89% aa sequence identity with mouse MYF-5.
• Applications:Suitable for use in Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.