MSH2 is a 100-106kD member of the mutS family of DNA mismatch repair molecules.
It is a DNA binding protein that is expressed in rapidly proliferating cells, and acts in concert with multiple subunits.
MSH2 forms a heterodimer with MSH6, forming MutS.
This dimer recognizes and repairs both nucleotide misparings and one or two aberrant nucleotide insertions/deletions.
MSH2 also heterodimerizes with MSH3, forming MutSB.
This complex acts on DNA double-stranded breaks, and repairs large nucleotide insertions/deletions (<15 bases).
MutSA is associated with BLM:p53:RAD51 complexes, while MSH2B is associated with SLX4/BTBD12 complexes.
Human MSH2 is 934aa in length.
It contains three MutS domains aa18-132; 158-284; 297-612 plus an ABC transporter signature motif that may hydrolyze ATP aa 633-852.
There are multiple splice variants.
One shows a premature truncation after His429, while others contain a 46aa substitution for aa879-934, a 29aa substitution for aa462-934, a 28aa substitution for aa783-934, a 3aa substitution for aa532-934, a 5aa substitution for aa482-934 and a 31aa substitution for aa379-934.
Over aa1-140, human MSH2 shares 94% aa identity with mouse MSH2.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human MSH2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa2-140 from human MSH2 expressed in E. coli.
