MP-19 (Matrix metalloprotease 19; also MMP-18 and MMP RASI) is a 55-59kD member of the peptidase M10A family of enzymes.
It is widely expressed, being secreted by stratum basale keratinocytes, smooth muscle cells, epiphysial cartilage chondrocytes and monocytes/macrophages.
MMP-19 has multiple substrates, including components of the basement membrane (type IV collagen; laminin; nidogen), fibronectin, aggrecan plus COMP, and IGFBP3, this latter cleavage resulting in the release of active IGF-I.
Studies involving MMP-19 demonstrate an antiangiogenic function.
This is attributable to the processing of plasminogen, generating angiostatin-like molecules, and the creation of an environment that promotes the ECM retention of soluble VEGF.
Human proMMP-19 is 490aa in length.
It contains an autocleavable 9kD propeptide aa19-97 plus a 411aa mature region aa98-508.
The mature region contains a Zn catalytic region aa103-256 plus four hemopexin-like domains aa293-508.
There are three additional potential isoforms.
One shows an 88aa substitution for aa300-508, a second contains a12aa substitution for aa1-298, while a third possesses an alternative start site at Met80.
Over aa229-508, human MMP-19 shares 76%aa identity with mouse MMP-19.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human MMP-19.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa229-508 from human MMP-19 expressed in NSO cell line.
