84-1216-15　［取扱停止］LTK (Leukocyte Tyrosine Kinase Receptor, Protein Tyrosine Kinase 1, TYK1) 100ug　L4725-50D

特徴

• LTK is a glycoprotein member of the tyrosine protein kinase family, insulin receptor subfamily of proteins.
• There are multiple isoforms that run between 50 and 100kD in SDS-Page.
• It is reportedly expressed in lymphocytes and cerebral cortex neurons, plus cardiomyocytes where it plays a role in cellular hypertrophy.
• Mature human LTK is an 848aa type I transmembrane protein that is embedded in the ER.
• It contains a 408aa extracellular domain aa17-424 plus a 415aa cytoplasmic region aa450-864.
• The cytoplasmic region possesses multiple phosphotyrosines that interact with downstream signaling molecules.
• There is also a protein kinase domain between aa510-786.
• LTK apparently undergoes dimer- and trimerization under certain circumstances.
• There are multiple splice variants for LTK.
• Two isoforms possess a common deletion of aa274-334 that may be accompanied by a 27aa substitution for aa449-544.
• Another isoform shows an alternative start site at aa406 accompanied by a 17aa substitution for aa449, while a fourth isoform contains a 51aa substitution for aa171-864.
• A final 50kD isoform shows a 28aa substitution for aa449-864.
• Over aa17-424, human LTK shares 75% aa identity with mouse LTK.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.