Reg1A is a secreted, variably glycosylated 15-22kD type I member of the REG gene family of proteins.
It is induced in proliferating cell types such as colonic epithelium, islet B-cells, and multiple tumor types, and is constitutively expressed by pancreatic acinar cells.
Thus, Reg1A is found both in blood (ng/ml quantities) and pancreatic secretions.
It would appear that Reg1A plays a role in blocking apoptosis, and is upregulated by both IL-22 and IL-6.
Human Reg1A is synthesized as a 166aa precursor.
It contains a 22aa signal sequence plus a 144aa mature region that is characterized by the presence of three intrachain disulfide bonds, one C-type lectin domain aa34-164 and a utilized N-terminal O-linked glycosylation site 27aa.
Variablility in the glycosylation pattern, plus proteolytic cleavage after 11aa generates Reg1A isoforms in the 15-22kD range.
There is one potential isoform variant that shows an alternative start site at Met9.
Mature aa23-166 human Reg1A shares 76% and 69%aa sequence identity with mouse and rat Reg1A, respectively.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human Reg1A. Species sequence homology: recombinant human Reg1B (80%), recombinant mouse Reg1 (20%), rhReg3A and recombinant rat Reg1A (5%).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa23-166 from human Reg1A.expressed in CHO cell line.
