Glycoprotein 130 is a ubiquitously expressed, 130kD type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family.
Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane.
Rat gp130 is synthesized as a 918aa precursor with a 22aa signal sequence, a 596aa extracellular domain (ECD), a 22aa transmembrane region, and a 278aa cytoplasmic tail.
Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD.
The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains.
The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family.
Rat gp130 shares 88% and 79% aa sequence identity with mouse and human gp130, respectively.
Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1, IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC).
These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes rat GP130. Species Crossreactivity: Recombinant mouse gp130 (~45%), recombinant human gp130 (~6%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to aa23-618 from rat GP130 expressed in NSO cell line.
