84-1216-06　［取扱停止］ITGB1BP1 (ICAP1, Integrin beta-1-binding Protein 1, Integrin Cytoplasmic Domain-associated Protein 1, ICAP-1) 100ug　I7661-38X1

特徴

• ICAP-1 is a 22-27kD protein that specifically binds to the cytoplasmic tail of B1 integrin.
• It is widely expressed, and serves to negatively regulate cell migration/proliferation.
• In the cytosol, it has at least two functions.
• First, it associates withz B1 integrin, promoting an inactive conformation.
• This has the effect of both slowing cell migration and allowing for the monitoring of ECM density.
• Second, it induces Delta-Notch signaling, an event that specifies the growth of only tip endothelium during angiogenesis.
• In the nucleus, ICAP-1 is proposed to participate in c-myc activation.
• Human ICAP-1 is 200aa in length.
• It contains an NLS aa5-9 and one pleckstrin homology-like/PID domain aa58-200.
• Phosphorylation occurs at aa38-41.
• There is one nonintegrin-binding alternative splice form that shows a deletion of aa128-177.
• Over aa1-127, human ICAP-1 shares 93% aa identity with mouse ICAP-1.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and StabilityLyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.