84-1216-03　HGF (Hepatocyte Growth Factor, Hepatopoietin-A, Scatter Factor, SF) (Biotin) 50ug　H2005-08D

特徴

• HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases.
• It is a multidomain molecule that includes an N-terminal PAN/APPLE like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity.
• Canine HGF is secreted as an inactive 699aa single chain propeptide.
• It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide linked HGF with a 60kD A and 30kD B chain.
• A variant of HGF with a 5aa deletion in the first Kringle domain has been described in canine and other species.
• Canine HGF shares 97%, 99%, 93%, 93%, and 89% aa sequence identity with bovine, feline, human, mouse, and rat HGF, respectively.
• HGF binds heparan sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGR/cMET.
• HGF dependent cMET activation is implicated in the development of many human cancers.
• HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis.
• HGF induces the upregulation of integrin A2B1 in epithelial cells by a selective increase in A2 gene transcription.
• This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis.
• HGF can also alter epithelium morphology by the induction of nectin-1a ectodomain shedding, an adhesion protein component of adherens junctions.
• In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSHs-timulated iodine uptake.
• HGF promotes the motility of cardiac stem cells in damaged myocardium.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1mg/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile Tris, pH 7.3 (20mM Trizma base, 150mM sodium chloride), 0.1% BSA.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.