GDF-3 is a TGF-B superfamily member belonging to the growth/differentiation factor family.
GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, adipose tissue and the brain.
In ES cells, it maintains pluripotency and influences early cell fate decisions.
For example, frog embryos injected with GDF-3 develop a secondary dorsal axis and deletion of mouse GDF-3 can produce defects in the anterior visceral endoderm of the pre-gastrulation embryo.
In adipocytes, GDF-3 is induced by a high fat diet and promotes adipogenesis.
GDF-3 has been reported to oppose BMP’s functions and to have a nodal-like activity in early development.
The 366aa mouse GDF-3 contains a 22aa signal sequence, a 230aa propeptide and a 114aa mature protein that contains one potential N-glycosylation site.
Most of GDF-3 is present as the prepro form, while the mature GDF-3 is presumably the secreted, active form.
The mature protein contains the cysteine-knot structure that is conserved throughout family members.
Since it lacks the fourth cysteine, which is responsible for the formation of inter-molecular disulfide bond, GDF-3 may exist as a non-covalent homodimer.
Mature mouse GDF-3 shares 90%, 83%, and 83% aa identity with rat, human and canine GDF-3, respectively.
Among family members, mature GDF-3 is most similar to mouse BMP-6 (45% aa identity) and Xenopus VG-1(52% aa identity).
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1.5ug/mlImmunohistochemistry: 5-15ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human GDF-3. Species Crossreactivity: mouse. Species sequence homology: Recombinant mouse GDF-5, rmGDF-6, rmGDF-7, rmGDF-8, and rmGDF-9 (5%).
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant corresponding to aa253-366 from human GDF-3 expressed in E.coli.
