Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes and natural killer (NK) cells.
Granzyme A is the most abundant protease in CTL and NK cells.
It induces caspase-independent cell death when introduced into target cells by perforin.
Human granzyme A is synthesized as a 262aa precursor with a signal peptide aa1-6, a propeptide aa27-28 and a mature chain aa29-262.
After being activated by lysyl endopeptidase removal of the propeptide, Granzyme A cleaves thioester substrates.
Mature human granzyme A shares 71% aa sequence identity with mouse granzyme A.
Applications:Suitable for use in Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human Granzyme A.
Isotype：IgG
Calc Applications Abbrev：IC
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa26-262 from human Granzyme A expressed in NSO cell line.
