GAPDS is a 40-44kD member of the GAPDH family of enzymes.
Due to a high incidence of proline residues, it may migrate anomalously at 56kD in SDS-PAGE.
GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath.
Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility.
Human GAPDH-2 is 408aa in length.
There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa223-226.
One splice variant shows a 9aa insertion after Ser220.
Over aa75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human GAPDHS. Species sequence homology: recombinant human GAPDH and recombinant mouse GAPDH (~1%).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa75-222 from human GAPDHS expressed in E. coli.
