FSTL4 is a likely secreted, 90kD (predicted) member of the follistatin gene family of TGF-B superfamily inhibitors.
It is widely expressed, being found in neurons (retinal ganglion and cerebellar Purkinje cells), cardiac muscle cells, smooth muscle cells and intestinal epithelium.
Mature mouse FSTL4 is 819aa in length aa23-841.
It contains one kazal-like domain aa80-134, an EF-hand motif aa173-208, and two Ig-like domains aa250-336 and 340-425.
There are two potential splice forms, one that shows an alternative start site at Met315, and a second that possesses a His residue substitution for aa518-841.
Full-length mature mouse FSTL4 shares 95% and 84% aa identity with rat and human FSTL4.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes recombinant and native mouse FSTL4. Species Crossreactivity: human and rat. Species sequence homology: Recombinant human (10%), rhFSTL5 and recombinant mouse FSTL1 (5%).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant corresponding to aa23-841 from mouse FSTL4 expressed in CHO cell line.
