84-1215-89　FGF15 (Fibroblast Growth Factor 15, FGF-15) 100ug　F4212-20

特徴

• FGF-15 is a 25kD, secreted, nonglycosylated polypeptide that is a member of the FGF-15/21/23 subfamily, FGF family of growth factors.
• It is synthesized by ileal epithelium, colonic myofibroblasts, and select areas of the developing brain.
• FGF-15 acts on hepatocytes and reduces CYP7A1 levels, thus blocking bile acid production.
• It also promotes colonic epithelial migration, and in the embryonic brain, inhibits over-production of neuronal precursors.
• FGF-15 is synthesized as a 218aa precursor that contains a 25aa signal sequence and a 182aa mature region aa26-218; 23kD predicted MW).
• Mature FGF-15 contains a characteristic 3-dimensional B-trefoil structure, plus a unique Cys pattern that contributes to stability.
• There are two potential splice forms.
• One shows a 23aa substitution for aa120-218, while a second shows a 41aa substitution for aa120-218.
• Over aa23-200, mouse FGF-15 shares 96% aa identity with rat FGF-15.
• Although mouse FGF-15 is considered the ortholog to human FGF-19, the two molecules share only 54% aa identity over aa23-200, and are produced by different cell types (intestinal epithelium in mouse, hepatocytes in human).
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of monkey embryoOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.