Fc Receptor-Like 5 (FCRL5), also known as FcRH3 (FcRH5 in human), IRTA2, and CD307e, is a 90-95kD member of the FCRL family of proteins whose aa sequence is reminiscent of classical Fc receptors.
FCLR molecules are type I transmembrane proteins that contain from three to nine Immunoglobulin-like domains.
They are differentially expressed within the B cell lineage, and can either promote or inhibit B cell proliferation and activation.
Mature mouse FCRL5 consists of a 470aa extracellular domain (ECD), a 21aa transmembrane segment, and a 79aa cytoplasmic region.
FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood, and is particularly noted to be expressed on T-independent marginal zone and B1 B cells.
Its ligation inhibits signaling through the B cell antigen receptor.
Epstein-Barr virus transformation of B cells induces the up-regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription.
FCRL5 on B cells functions as a receptor for the orthopoxvirus MHC class I-like protein OMCP.
Based on the literature and testing, both mouse and human FCRL5 will bind to purified IgG with high affinity.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 10ul labels up to 1x10e6 cellsOptimal dilutions to be determined by the researcher.
Absorption:620-650nm
Emission:660-670nm
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes mouse FCRL5. <1% cross-reactivity is observed with recombinant human FCRL5 in dIrect ELISA.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln27-Ala496 from mouse FCRL5 expressed in NSO cell line.
