EBF-2 is a 62kD member of the COE family of transcription factors.
It is expressed in immature osteoblasts and Purkinje cells, and in the embryo is associated with the migration of postmitotic neuroblasts.
In immature osteoblasts, EBF-2 appears to upregulate OPG, suppressing osteoclast formation.
And in the developing retina, EBF-2 is found in ganglion, glycinergic Amacrine and horizontal cells, possibly promoting their development over that of photoreceptor cells.
Mouse EBF-2 is 575aa in length.
It contains one DNA-binding region with an embedded C5-type zinc-finger motif aa62-238, a dimerization ITP/TIG domain aa253-336, and a Pro/Ser-rich transactivation domain aa453-534.
Although considered an HLH type transcription factor, it does not contain the typical "b", or basic aa sequence associated with bHLH factors.
EBF-2 both homodimerizes, and heterodimerizes with EBF-1 and-3.
There is an alternative start site at Met23.
Over aa407-519, mouse EBF-2 is identical in aa sequence to rat EBF-2 and shares 99% aa identity with human EBF-2.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes mouse EBF-2. Species sequence homology: Recombinant mouse EBF-1 and rmEBF-3 (1%).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to aa407-519 from mouse EBF-2 expressed in E.coli.
