Dystroglycan, also DAG-1 is a 180-200kD heterodimeric adhesion molecule that links the cell cytoskeleton to the extracellular matrix.
It is found on skeletal muscle, cardiac muscle, fibroblasts, smooth muscle and keratinocytes.
DAG-1 binds multiple matrix molecules, including laminin-1 and -2, agrin, and perlecan.
Intracellularly, the cytoplasmic tail of DAG-1 contributes to a large 400kD complex that interacts with the cytoskeleton.
The human DAG-1 preprocursor is a type I transmembrane protein 895aa in length.
It contains a 27aa signal sequence plus an 868aa proform that undergoes autocatalysis to generate a 626aa A-chain aa28-653 and a 242aa B-chain.
Mature DAG-1 is a heterodimer composed of noncovalently linked A-and B-chains.
The A-chain possesses one potential Ig-like domain aa64-162, a mucin-like region aa316-485, and a peptidase S72 domain aa500-733 It is O-glycosylated and runs from 100-160kD in SDS-PAGE.
The B-chain is N-glycosylated and runs at 42-44kD in SDS-Page.
It possesses a short 95aa extracellular region aa654-749 plus a 120aa cytoplasmic domain aa776-895.
Membrane cleavage of the B-chain causes dissociation of the heterodimer and generates a 30kD truncated form.
Over aa28-749, human DAG-1 shares 93% aa identity with mouse DAG-1.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human Dystroglycan.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa28-479 from human Dystroglycan expressed in NSO cell line.
