84-1215-83　DCTN2 (Dynactin Subunit 2, Dynactin Complex 50kD Subunit, DCTN-50, 50kD Dynein-associated Polypeptide, p50 Dynamitin, DCTN50) 100ug　D9895-03A

特徴

• DCTN2 (Dynactin2; also 50kD dyneinassociated polypeptide and p50 dynamitin) is a 50kD intracellular member of the dynactin subunit 2 family of phosphoproteins.
• It is ubiquitously expressed and serves to stabilize the dynactin complex of 11 different proteins.
• This complex is an obligate cofactor of dynein and kinesin motors, which position the mitotic spindle for cell division, and move vesicles within the cell.
• Human DCTN2 is 401aa in length.
• It contains three coiled coil regions (aa99-132, 214-244 and 379-399), two potential phosphorylation sites (Ser83 and Tyr86), and one acetylation site at Ala2.
• The first two coiledcoil regions contribute to oligomerization, while aa191 are essential for dynactin regulation.
• Potential isoform variants utilize either a five or two aa insertion after Ala35, an alternative start site at Met88, and a 21aa substitution for aa118-401.
• Over aa277-401, human DCTN2 shares 95% aa identity with mouse DCTN2.
• Applications:Suitable for use in Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.