84-1215-80　Dsc2 (Desmocollin-2, Epithelial Type 2 Desmocollin, Dsc3) 100ug　D3221-51

特徴

• DSC2 (Desmocollin [Greek for "glue that binds"] 2; also epithelial type 2 desmocollin) is a 100-120kD member of the Ca++dependent cadherin family of adhesion molecules.
• It is found on the surface of both simple and stratified epithelium that form desmosomes.
• DSC2 expression is induced by DSG2 where it serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments.
• Homodimeric DSC2 forms intrans, and DSC 2 may also form heterotypic interactions with DSG2.
• A DSC2: DSG2 cis-interaction is suggested to block DSG2 activation of Akt, an action that results in B-catenin mediated cell proliferation.
• Mature mouse DSC2 is a 767aa type I transmembrane glycoprotein (aa136-902).
• The mature molecule contains a 559aa extracellular region (aa136-694) with 5 cadherin domains (aa136-694) and a 187aa cytoplasmic tail (aa716-902).
• There is one 100-110kD splice variant that shows an 11aa substitution for aa838-902.
• Over aa136-694, mouse DSC2 shares 87% and 79% aa sequence identity with rat and human DSC2, respectively.
• Applications:Suitable for use in Immunohistochemistry and Direct ELISA.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: 5-15ug/ml using perfusion fixed frozen sections of mouse embryoOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.