CAMP (Cathelicidin AntiMicrobial Peptide; also18kD cationic antimicrobial protein, CAP18, LL37, FALL39 and HSD26) is a member of the cathelicidin family of proteins.
It is widely expressed, being found associated with neutrophils, bronchial epithelium, renal tubule epithelium, activated keratinocytes, gd T cells, monocytes, NK cells, colonic epithelium and the stratum basale of nonkeratinized epithelium found in the vagina and oral cavity.
CAMP has marked antimicrobial activity against both Gm+ and Gm- bacteria, and acts as a chemoattractant for neutrophils, monocytes and mast cells.
CAMP is synthesized as 170aa preproprecursor.
It contains a 30aa signal sequence, a 103aa, 14kD prosegment (aa31-131) and a 4-5kD, 37aa (aa134-170) C-terminal mature fragment (LL37) or 39aa (aa132-170) C-terminal mature fragment (FALL39).
In neutrophils, the 18-19kD proprecursor is stored in granules, where, upon activation, it is enzymatically cleaved and released.
While both the prosegment and C-terminal fragments possess antimicrobial activity, the prosegment also shows protease activity, while the C-terminal fragment also shows chemotactic activity.
The prosegment may form homodimers, while the C-terminal fragment (LL37) is reported to form homotetramers.
Over aa141-170, human CAMP shares only 50% and 57% aa sequence identity with mouse and rat CAMP, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human CAMP.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa134-170 from human CAMP expressed in E.coli.
