84-1215-75　Contactin-1 (Neural Cell Surface Protein F3, Cntn1) 100ug　C7865-19B

特徴

• CNTN1 (Contactin1; also neuronal cell surface protein F3/F11) is a 135-145kD GPI-linked glycoprotein member of the Contactin family, Ig superfamily of molecules.
• It is expressed on the surface of oligodendrocytes and neurons, principally on axons, and has multiple binding partners.
• In particular, it binds to astrocyte tenascin C, Schwann cell NF/neurofascin155, and oligodendrocyte produced tenascin-R and Notch intrans, plus paranodin in cis.
• Tenascin-R interactions inhibit neurite outgrowth, paranodin/NF155 interactions may contribute to Ranvier node development, and Notch ligation results in oligodendrocyte maturation.
• CNTN1 also binds to chondroitin sulfate E, an interaction that initiates neurite outgrowth.
• Mouse CNTN1 is synthesized as a 1020aa preproprecursor (aa1-1020).
• It contains a 20aa signal sequence, followed by a 981aa mature region (aa21-1001), and a 19aa propeptide.
• The mature region possesses six consecutive C2-type Ig-like domains (aa41-603) that regulate neurite outgrowth, followed by three FN-type III domains (aa605-901) that regulate adhesiveness.
• It is estimated that 15% of the native MW constitutes carbohydrate, part of which is represented by the HNK 1 antigen (3sulfo-GluA-Gal-GlcNac).
• Over aa20-999, mouse CNTN1 shares 96% and 99% aa sequence identity with human and rat CNTN1, respectively.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.