Collagen XIII A1 is an 85-95kD protein in the type 2 transmembrane collagen family.
Mature mouse Collagen XIII A1 consists of a 40aa cytoplasmic domain, a 19aa transmembrane segment, and a 692aa extracellular domain (ECD).
The ECD contains three collagenous regions separated by shorter non-collagenous regions.
Within comparable regions of the ECD, mouse Collagen XIII A1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII A1, respectively.
Mouse Collagen XIII A1 is extensively spliced, with some isoforms showing a tissue specific distribution.
Collagen XIII A1 is widely expressed during development and in the adult.
It localizes to intercellular adherens junctions and cell-matrix focal adhesions.
Collagen XIII A1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation.
Trimerization involves triple helix formation within the collagenous domains, although portions of the non-collagenous regions can also form coiled coils.
The ECD of trimeric Collagen XIII A1 is an extended rod-like structure with two flexible hinges that correspond to non-collagenous regions.
Collagen XIII A1 clusters in cholesterol-rich domains on the plasma membrane, and it can be cleaved from the cell surface or intracellularly by a furin-like protease.
Collagen XIII A1 binds the extracellular matrix molecules fibronectin, heparin, integrin A1 nidogen-2, perlecan.
The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml detects Collagen XIII A1 in lysates of HT1080 human fibrosarcoma cell lineImmunohistochemistry: 5-15ug/ml in immersion fixed frozen sections of mouse embryo (13 d.p.c.)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Collagen XIII A1. Shows ~ 35% cross-reactivity with recombinant human (rh) COL13A1v4 is observed, and less than 1% cross-reactivity with rhCOL4A1, rhCOL25A1, rhCOL3A1, and rhCOL1A1
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu107-Gln565 from mouse Collagen XIII A1, expressed in NSO cell line.
