CHD-1L is a 98-118kD member of the SNF2/RAD54 helicase family of proteins.
It is expressed in hepatocytes, possesses ATPase activity, and likely promotes chromatin remodeling at sites of DNA damage.
It also is considered an oncogene.
CHD-1L upregulates ARHGEF9 trabscription, an action that promotes Cdc42 activity with accompanying filopodia formation and EMT.
It also binds apoptosis-mediating Nur77, blocking its migration from nucleus-to-mitochondria.
Human CHD-1L is 897aa in length.
It contains a helicase ATP-binding domain aa58-223, a C-terminal helicase domain aa351-513, a coiled-coil region aa38-675 and one Macro domain that binds poly-ADP-ribose and targets DNA damage sites aa704-897.
There are at least two Ser/Thr phosphorylation sites.
There are multiple potential splice variants.
One isoform contains an alternative start site at Met114, a second isoform possesses a deletion of aa43-246, a third isoform shows a three aa substitution for aa363-897, and a fourth isoform combines a 16aa insertion after 386aa with a five aa substitution for aa425-897.
Over aa759-879, human CHD-1L shares 94% aa identity with mouse CHD-1L.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human CHD1L. Species sequence homology: Recombinant human CHD1 and rhCHD5 (3%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa759-879 from human CHD1L expressed in CHO cell line.
